NANOG antibody

• Catalog Number: orb19063
• Category: Antibodies
• Description: Goat polyclonal antibody to NANOG
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Porcine
• Dilution range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml
• Conjugation: Unconjugated
• MW: 34.6
• Target: NANOG
• Entrez: 79923
• Protein Sequence: QNQRMKSKRWQKNN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NANOG antibody, anti Nanog homeobox antibody,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 μg/ml.Experiment Notes: Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2;460(7251):53-9. Epub 2009 May 31. (PMID: 19483674). This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Ovary lysate using NANOG antibody

Immunofluorescence analysis of Human Keratinocytes using NANOG antibody