N-sulfated heparan sulfate antibody

• Catalog Number: orb623572
• Category: Antibodies
• Description: Mouse monoclonal antibody to N-sulfated heparan sulfate
• Clonality: Monoclonal
• Clone Number: HepSS-1
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: All
• Isotype: Mouse IgM
• Immunogen: MethA murine fibrosarcoma
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 3-12 μg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: N-sulfated heparan sulfate
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-N-sulfo-rich heparan sulfate antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 3-12 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of HUVEC cells stained using anti-N-sulfated heparan sulfate (HepSS-1) purified antibody (concentration in sample 9 μg/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).