N-sulfated heparan sulfate antibody (PE)

• Catalog Number: orb623595
• Category: Antibodies
• Description: Mouse monoclonal antibody against N-sulfated heparan sulfate conjugated to PE
• Clonality: Monoclonal
• Clone Number: HepSS-1
• Tested applications: FC
• Reactivity: All
• Isotype: Mouse IgM
• Immunogen: MethA murine fibrosarcoma
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: N-sulfated heparan sulfate
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-N-sulfo-rich heparan sulfate antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of EA.hy926 cells (red-filled) from EA.hy926 cells treated with heparinase (1 hour, 1 UI per ml of cell suspension) stained using anti-N-sulfated heparan sulfate (HepSS-1) PE antibody (concentration in sample 10 μg/ml).