N-cadherin (Extracellular) Antibody

SKU: orb1294388

Description

Rabbit polyclonal antibody to N-Cadherin

Images & Validation

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Item 1 of 7

Tested Applications	FC, IF, IHC, WB
Dilution range	Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:200-1:300 Flow Cytometry 1:50-1:100
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Extracellular domain corresponding to Human N-cadherin
Target	N-cadherin (Extracellular)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: N-cadherin antibody (orb1294388).

Cell: H1299, Phalloidin: 1-300, N-cadherin (orb1294388): 1-200, Anti-rabbit 488: 1-300, NikonEpi-fluorescence (A-ken) 60x.

Immunohistochemical analysis of paraffin embedded Human breast and kidney cancer tissue labeling N-cadherin with orb1294388 at 1/100.

Immunohistochemical analysis of paraffin embedded Human lymph node cancer tissue labeling N-cadherin with orb1294388 at 1/100.

Immunohistochemical analysis of paraffin embedded Human testis cancer tissue labeling N-cadherin with orb1294388 at 1/100.

N cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294388 overnight at 4°C. E-cadherin antibody (orb1294381).

N-cadherin antibody, orb1294388, Cell: A549, N-cadherin (orb1294388): 1-100, Anti-rabbit 488: 1-1000.

Quick Database Links

Gene Symbol

N-cadherin (Extracellular)

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence