N-cadherin (Extracellular) Antibody

• Catalog Number: orb1294388
• Category: Antibodies
• Description: Rabbit polyclonal antibody to N-Cadherin
• Target: N-cadherin (Extracellular)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human N-cadherin
• Tested applications: FC, IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:200-1:300 Flow Cytometry 1:50-1:100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: N-cadherin antibody (orb1294388).

Cell: H1299, Phalloidin: 1-300, N-cadherin (orb1294388): 1-200, Anti-rabbit 488: 1-300, NikonEpi-fluorescence (A-ken) 60x.

Immunohistochemical analysis of paraffin embedded Human breast and kidney cancer tissue labeling N-cadherin with orb1294388 at 1/100.

Immunohistochemical analysis of paraffin embedded Human lymph node cancer tissue labeling N-cadherin with orb1294388 at 1/100.

Immunohistochemical analysis of paraffin embedded Human testis cancer tissue labeling N-cadherin with orb1294388 at 1/100.

N cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294388 overnight at 4°C. E-cadherin antibody (orb1294381).

N-cadherin antibody, orb1294388, Cell: A549, N-cadherin (orb1294388): 1-100, Anti-rabbit 488: 1-1000.