Myoglobin Rabbit Polyclonal Antibody

• Catalog Number: orb500695
• Category: Antibodies
• Description: Myoglobin Rabbit Polyclonal Antibody
• Target: MB
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Purified Human Myoglobin (Natural Human Myoglobin protein)
• UniProt ID: P02144
• MW: 17 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MYO; MB; MGC13548; PVALB; MYG_HUMAN.
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (orb500695) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (orb500695) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Human Myoglobin Protein at 100 ng, Muscle (Mouse) Lysate at 30 ug, Muscle (Rat) Lysate at 20 ug, Primary: Anti-Myoglobin (orb500695) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 14 kD.

Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Primary: Anti-Myoglobin (orb500695) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD.