MYL3 Rabbit Polyclonal Antibody

• Catalog Number: orb6428
• Category: Antibodies
• Description: MYL3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3 (101-195/195aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: MYL3
• UniProt ID: P08590
• RRID: AB_10938642
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cardiac myosin light chain 1; Slow skeletal Myosin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Rat Muscle tissue lysates, Primary: Anti-MYL3 (orb6428) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 25 kDa.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated (orb6428) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated (orb6428) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.