MYL3 Rabbit Polyclonal Antibody

SKU: orb6428

Description

MYL3 Rabbit Polyclonal Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3 (101-195/195aa)
• Target: MYL3
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMH8; MLC-lV/sb; MLC1SB; MLC1V; VLC1; VLCl; MLC1s; Mylc; MLClV; Mylc1v; rVMLC1; MYL3_HUMAN; MYL3; Cardiac myosin light chain 1 (CMLC1); Myosin light chain 1, slow-twitch muscle B/ventricular isoform (MLC1SB); Ventricular myosin alkali light chain; Ventricular myosin light chain 1 (VLCl); Ventricular/slow twitch myosin alkali light chain (MLC-lV/sb); MYL3_MOUSE; Myosin alkali light chain 1, ventricular/slow skeletal muscle isoform; MYL3_RAT; Myosin alkali light chain 1, ventricular (MLClV); Ventricular myosin light chain 1 (rVMLC1);

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Rat Muscle tissue lysates, Primary: Anti-MYL3 (orb6428) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 25 kDa.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated (orb6428) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated (orb6428) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MYL3

UniProt

P08590