MYH7 Rabbit Polyclonal Antibody

SKU: orb1206

Description

MYH7 Rabbit Polyclonal Antibody

Research Area

Autoimmune, Cytokinesis, Immunology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Canine, Human, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MYH7 (1301-1500/1935aa)
• Target: MYH7
• Molecular Weight: 213 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MYH7_HUMAN; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta; MYH7; MYHCB; CMH1; MPD1; SPMD; SPMM; CMD1S; CMYO7A; CMYO7B; CMYP7A; CMYP7B;

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (orb1206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MYH7B) Polyclonal Antibody, Unconjugated (orb1206) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (orb1206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (orb1206) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Quick Database Links

Gene Symbol

MYH7

UniProt

P12883