Mutant p53 (N235K, N239Y) Rabbit Polyclonal Antibody

SKU: orb11211

Description

Mutant p53 (N235K, N239Y) Rabbit Polyclonal Antibody

Research Area

Cancer Research, Tumor Suppressors, p53 Signaling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P53 arund the Mutation site N235K N239Y (230-241/393aa)
• Target: TP53
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Mtp53 (N235K N239Y) antibody (orb11211), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mtp53 (N235K N239Y)) Polyclonal Antibody, Unconjugated (orb11211) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Tissue/Cell: Hela, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Mtp53 (N235K N239Y)) Polyclonal Antibody, Unconjugated (orb11211) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

Abhijit Basu 1, Peters Thorsten 2, Björn Schumacher 3, Dimitris Kletsas 4, Karin Scharffetter-Kochanek 5 2 The dark side of the light (UVA): melanoma microenvironment and cell survival strategies Cell Death Discov, (2025)

PubMed: 41115909

Applications

WB

Reactivity

Mouse