MUSK Antibody

• Catalog Number: orb1263028
• Category: Antibodies
• Description: MUSK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This MUSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human MUSK.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 97 kDa
• Target: MUSK
• UniProt ID: O15146
• NCBI: O15146
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Muscle, skeletal receptor tyrosine-protein kinase,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MUSK Antibody in Jurkat cell line lysates (35 ug/lane)

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MuSK protein expression in extracts of COS cells after transfection with MuSK mutated and GFP constructs. WB with polyclonal MuSK and monoclonal GFP antibodies showed normal expression of the wild-type MuSK protein (WT), diminished expression of the GA mutant MuSK and no expression of the insC mutant or the pcDNA3 vector alone in transfected COS cells. GFP cotransfection was used to verify transfection efficiency.

HA-tagged MuSK and GFP constructs were transfected into C2C12 cells. Transfected C2C12 cells were treated with cycloheximide (CHX) and at different times after addition of CHX, amounts of WT and mutant MuSK were analyzed by WB with anti-HA antibody. Alpha-tubulin was used as an internal control and transfection efficiency was verified with anti-GFP antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.