Muscle Glycogen Synthase (phospho-S641) antibody

• Catalog Number: orb345666
• Category: Antibodies
• Description: Muscle Glycogen Synthase (phospho-S641) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:1,000 - 1:5,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS641 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P13807
• NCBI: NP_001155059.1
• Storage: Store Phospho GSY1 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-muscle glycogen synthase pS641 antibod
• Note: For research use only
• Application notes: This phospho specific polyclonal antibody was tested by immunohistochemistry, immunoblotting, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Prostate tissue using Muscle Glycogen Synthase (phospho-S641) antibody

Western blot analysis of human muscle tissue using Muscle Glycogen Synthase (phospho-S641) antibody