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Catalog Number | orb223861 |
---|---|
Category | Proteins |
Description | Recombinant Murine LIF protein |
Tested applications | SDS-PAGE |
Purity | > 98% by SDS-PAGE and HPLC analyses. |
Conjugation | Unconjugated |
MW | 19.9 kDa |
Target | Murine LIF |
Source | E. coli |
Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 iN 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50% of the M1 colonies in 1 ml agar cultures. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Murine LIF protein
Unconjugated | |
95% | |
25.8 kDa | |
Human Oncostatin M Protein, premium grade (orb257725) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 252 (Accession # NP_065391.1). |
Unconjugated | |
90% | |
22.2 kDa | |
Human Oncostatin M (OSM) (26-221), Tag Free (orb1496309) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 221 (Accession # P13725). |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses | |
18.2 kDa |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses | |
20.3 kDa |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses | |
24.3 kDa |
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