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MUC5AC Antibody

Catalog Number: orb2637853

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2637853
CategoryAntibodies
DescriptionThis antibody recognizes the peptide core of gastric mucin M1 (>1,000kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Species/HostMouse
ClonalityMonoclonal
Clone Number45M1
Tested applicationsIHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenM1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis MUC5AC antibody is available for research use only.
Entrez4586
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
MUC5AC Antibody

IHC testing of FFPE human stomach stained with MUC5AC antibody (clone 45M1).

MUC5AC Antibody

IHC testing of FFPE human gastric carcinoma stained with MUC5AC antibody (clone 45M1).

MUC5AC Antibody

IHC testing of FFPE rat stomach stained with MUC5AC antibody (clone 45M1).

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