MUC1 Antibody

• Catalog Number: orb1252645
• Category: Antibodies
• Description: MUC1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MUC1/967
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MUC1
• UniProt ID: P15941
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MC
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human ovarian cancer tissue.

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human endometrial cancer tissue.

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human breast cancer tissue.