MUC1 Antibody

• Catalog Number: orb1252644
• Category: Antibodies
• Description: MUC1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MUC1/845
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this EMA antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.1. No pretreatment needed for FFPE staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MUC1
• UniProt ID: P15941
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MC
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.1. No pretreatment needed for FFPE staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).

SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.