MUC1 Antibody

• Catalog Number: orb1252643
• Category: Antibodies
• Description: MUC1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MUC1/955
• Tested applications: IHC
• Reactivity: Human, Mouse
• Isotype: IgG1, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this MUC-1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MUC1
• UniProt ID: P15941
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MC
• Note: For research use only
• Application notes: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human endometrial carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lung carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.