mTOR antibody

• Catalog Number: orb393116
• Category: Antibodies
• Description: Rabbit polyclonal antibody to mTOR.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Sheep
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mTOR. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MTOR
• Entrez: 56717, 56718, 2475
• UniProt ID: P42345, Q9JLN9, P42346
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti FK506 binding protein 12 rapamycin associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mTOR expression in HEK293T (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 288 kD; Observed band size: 289 kD)

Immunohistochemical analysis of mTOR staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.