MSR1 Rabbit Polyclonal Antibody

• Catalog Number: orb100585
• Category: Antibodies
• Description: MSR1 Rabbit Polyclonal Antibody
• Target: MSR1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human MSR1/CD204 (211-320/451aa)
• UniProt ID: P21757
• MW: 50 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD204; SP-A; CD204 antigen; Macrophage acetylated
• Note: For research use only

Blank control (Blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-MSR1 antibody (orb100585), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Liver (Mouse) Lysate at 40 ug, Lane 4: Placenta (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Mouse) Lysate at 40 ug, Lane 6: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 7: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/39 kD, Observed band size: 50 kD.

Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/cell: mouse testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.