MSH2 Antibody

• Catalog Number: orb2636733
• Category: Antibodies
• Description: Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rMSH2/6548
• Tested applications: IHC-P, WB
• Reactivity: Canine, Feline, Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human MSH2 protein was used as the immunogen for the recombinant MSH2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant MSH2 antibody is available for research use only.
• UniProt ID: P43246
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant MSH2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HCT-116 cell lysate using recombinant MSH2 antibody (clone rMSH2/6548). Expected molecular weight ~105 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant MSH2 antibody (clone rMSH2/6548) as confirmation of integrity and purity.