MSH2 Antibody

• Catalog Number: orb234330
• Category: Antibodies
• Description: MSH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MSH2 recombinant protein (Position: Q337-N583). Human MSH2 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat MSH2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 104743 MW
• UniProt ID: P43246
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA mismatch repair protein Msh2;hMSH2;MutS protei
• Note: For research use only
• Application notes: WB: The detection limit for MSH2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-MSH2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MSH2 using anti-MSH2 antibody.Lane 1:human HEK293 cell;2:human HEK293 cell;3:human HeLa cell;4:human COLO-320 cell;5:human T-47D cell;6:human A549 cell.

IF analysis of MSH2 using anti-MSH2 antibody.MSH2 was detected in immunocytochemical section of U20S cell.

IHC analysis of MSH2 using anti-MSH2 antibody.MSH2 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of MSH2 using anti-MSH2 antibody.MSH2 was detected in immunocytochemical section of SMMC Cell.