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Catalog Number | orb1530181 |
---|---|
Category | Antibodies |
Description | MSH2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 875 and C-term |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MSH2 |
UniProt ID | P43246 |
NCBI | NP_000242.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | hMSH2; MutS protein homolog 2; mutS homolog 2, col Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse MSH2 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-MSH2 antibody orb1530181 at 0.1 µg/ml.
Detection of mouse MSH2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human MSH2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human MSH2 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-MSH2 antibody.
ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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