MSH2 antibody

• Catalog Number: orb1473895
• Category: Antibodies
• Description: Mouse monoclonal antibody to MSH2
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human MSH2. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/100 - 1/500)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MSH2
• Entrez: 4436
• UniProt ID: P43246
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: DNA mismatch repair protein Msh2; hMSH2; MutS prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MSH2 expression in 293 (A), Hela (B) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 104 kD)

Immunohistochemical analysis of MSH2 staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.