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Catalog Number | orb1151539 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to MSH2 |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | MSH2/6549R |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | Recombinant full-length human MSH2 protein was used as the immunogen for the MSH2 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This MSH2 antibody is available for research use only. |
UniProt ID | P43246 |
Storage | Aliquot the MSH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the MSH2 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of human HCT-116 cell lysate using MSH2 antibody (clone MSH2/6549R). Expected molecular weight ~105 kDa.
IHC staining of FFPE human colon tissue with MSH2 antibody (clone MSH2/6549R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with MSH2 antibody (clone MSH2/6549R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free MSH2 antibody (clone MSH2/6549R) as confirmation of integrity and purity.
ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |