MRPL28 Antibody

• Catalog Number: orb1265712
• Category: Antibodies
• Description: MRPL28 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This MRPL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the N-terminal region of human MRPL28.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: MRPL28
• UniProt ID: Q13084
• NCBI: Q13084
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: 39S ribosomal protein L28, mitochondrial, L28mt, M
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MRPL28 immunoreactivity is localized to Mitochondria significantly.