MRP2/ABCC2 Rabbit Polyclonal Antibody

• Catalog Number: orb501001
• Category: Antibodies
• Description: MRP2/ABCC2 Rabbit Polyclonal Antibody
• Target: ABCC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human MRP2 (485-615/1545aa)
• UniProt ID: Q92887
• MW: 174 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: multidrug resistance-associated protein2; ABC30; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MRP2/ABCC2 Polyclonal Antibody, Unconjugated (orb501001) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

The A549 (H) cells were fixed with 4% PFA (10 min at r.t.). Primary Antibody (green): Rabbit Anti-MRP2/ABCC2 antibody (orb501001): 1 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.