MRF/C11orf9 Rabbit Polyclonal Antibody

SKU: orb157899

Description

MRF/C11orf9 Rabbit Polyclonal Antibody

Research Area

DNA / RNA Binding, DNA and RNA Research, Neurogenesis, Neuroscience, Oligodendrocyte Marker, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MRF (401-500/1151aa)
• Target: MYRF
• Molecular Weight: 124 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C11orf9; Chromosome 11 open reading frame 9; Gm1804; gm98; KIAA0954; MGC10781; MRF; MRF_HUMAN; Myelin gene regulatory factor; Ndt80.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human U2OS cell lysates, Lane 3: Human A431 cell lysates, Primary: Anti-MRF/C11orf9 (orb157899) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kDa, Observed band size: 124 kDa.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-MRF/C11orf9 (orb157899) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-MRF/C11orf9 (orb157899) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated (orb157899) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MYRF