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Catalog Number | orb1269200 |
---|---|
Category | Antibodies |
Description | MPP3 Antibody |
Target | MPP3 |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3. |
UniProt ID | Q13368 |
MW | 66 kDa |
Tested applications | FC, IF, WB |
Application notes | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | MAGUK p55 subfamily member 3, Discs large homolog Read more... |
Note | For research use only |
NCBI | Q13368 |
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562, NCI-H292 cell line lysates (35 ug/lane).
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.