MPP3 Antibody (C-term)

• Catalog Number: orb1936963
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33005
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 66152 Da
• Target: This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3.
• UniProt ID: Q13368
• NCBI: NP_001923.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: MAGUK p55 subfamily member 3, Discs large homolog
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-MPP3 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MPP3 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

MPP3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

MPP3 Antibody (C-term) western blot analysis in K562, NCI-H292 cell line lysates (35 ug/lane). This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).