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Catalog Number | orb1151493 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to MPO |
Clonality | Recombinant |
Species/Host | Rabbit |
Isotype | Rabbit IgG, kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Purity | Protein A/G affinity |
Immunogen | A portion of amino acids 150-250 was used as the immunogen for the recombinant Myeloperoxidase antibody. |
UniProt ID | P05164 |
Tested applications | IHC-P, WB |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Application notes | Optimal dilution of the recombinant Myeloperoxidase antibody should be determined by the researcher. |
Antibody Type | Primary Antibody |
Clone Number | MPO/33R |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Storage | Aliquot the recombinant Myeloperoxidase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Hazard Information | This recombinant Myeloperoxidase antibody is available for research use only. |
Note | For research use only |
IHC staining of FFPE human spleen tissue with recombinant Myeloperoxidase antibody (clone MPO/33R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant Myeloperoxidase antibody (clone MPO/33R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human spleen tissue lysate using recombinant Myeloperoxidase antibody (clone MPO/33R). Expected molecular weight: 75-90 kDa (pro form).