Mouse VEGF120 Protein

• Catalog Number: orb257917
• Category: Proteins
• Description: Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
• Reactivity: Mouse
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 14.1 kDa
• Target: VEGF120
• Source: Mouse VEGF120 Protein, Tag Free (orb257917) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # AAB22254.1 ).
• Expression System: HEK293
• Biological Activity: Immobilized Mouse VEGF120, Tag Free at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag with a linear range of 8-31 ng/mL (QC tested).
• Expression Region: Ala 27 - Arg 146
• Endotoxins: 0.1 EU per μg
• NCBI: AAB22254.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Mouse VEGF protein

Line graph illustrates about the sensitivity range of Mouse VEGF protein using different concentrations

Immobilized Mouse VEGF120, Tag Free (Cat. No. orb257917) at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. orb1184768) with a linear range of 8-31 ng/mL (QC tested).