Mouse Transferrin protein

• Catalog Number: orb420186
• Category: Proteins
• Description: Mouse Transferrin protein
• Target: Trf
• Isotype: Transferrin
• Conjugation: Unconjugated
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 5.9 mg/ml
• Buffer/Preservatives: Preservative: None. Stabilizer: None. See application note.
• Purity: Mouse Transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Mouse Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Transferrin and anti-Mouse Serum.
• UniProt ID: Q921I1
• Tested applications: SDS-PAGE
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Application notes: Mouse Transferrin is a suitable protein for use as a control reagent in SDS, Western Blotting, and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa. Mouse Transferrin, when unopened, is stable at 4° C, but for extended use should be aliquoted and stored at -20° C in its undiluted form. If dilutions of Mouse Transferrin are required, they should be prepared just prior to use and not retained.
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse Transferrin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: HP, Tf, Tfn, hpx, Cd176, AI266983, MGC102653, apot
• Note: For research use only
• NCBI: AAH22986.1

Fate of a preformed transferrin corona in vivo. A, activity of 59Fe and 125I (1–120 min) in blood; B and C, activity of 59Fe and 125I in organs 120 min after i.v. injection of 59Fe-SPIOs with a preformed protein corona of adsorbed (A, B right side) or covalently bound (A, B, left side) 125I-mouse-transferrin. mouse transferrin (p/n orb420186).

SDS-Page of Mouse Transferrin Lane 1: Mouse Transferrin Load: 1.0 µg per lane Predicted/Observed size: 80 kDa, 80 kDa.

The addition of C3 or fibronectin to QF enhanced hepatic transgene expression. Lipoplex was incubated with each component for 5 min before intraportal injection. QF, QAE column flow through fraction; C3, complement component C3; Fet, fetuin; AF, asialofetuin; FN, fibronectin; Tf, transferrin. Each bar represents the mean SE of at least seven experiments. *p < 0.05, **p < 0.01 versus QF group. Mouse transferrin (Tf) (p/n orb420186).