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Mouse TRAIL Protein
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg in the presence of actinomycin D. |
| Protein Sequence | MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Storage & Handling
−| Storage | Stability: Lyophilized TRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -1°C. Upon reconstitution TRAIL recombinant should be stored at 4C between 2-7 days and for future use below -1°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
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| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | The protein was lyophilized containing PBS, pH 7.4, and 3mM DTT. |
| Disclaimer | For research use only |
Alternative Names
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.5 kDa after removal of the signal peptide.
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Protocol Information
Mouse TRAIL Protein (orb428977)
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