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Mouse TNF a Protein

Mouse TNF a Protein

Catalog Number: orb428882

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb428882
CategoryProteins
DescriptionRecombinant of mouse TNF a protein
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 97.0% as determined by:(a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE.
Solubility (25°C)It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
SourceEscherichia Coli
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.1ng/ml, corresponding to a Specific Activity of 10,000,000 Units/mg.
StorageStability: Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Buffer/PreservativesLyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2.
Alternative namesTNF-alpha, Tumor necrosis factor ligand superfamil
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NoteFor research use only
Application notesProtein content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard
Expiration Date6 months from date of receipt.
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    ICC,  IF,  IHC

    Human, Mouse, Rat

    Mouse

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    100 μg
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    ELISA,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

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