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Mouse SPOCK3 protein

SKU: orb392515

Description

Recombinant mouse SPOCK3 protein is produced by our mammalian expression system and the target gene encoding Ala23-Ile436 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityMouse
Protein SequenceAAAVAVAGGR SDGGNFLDEK QWLTTISQYD KEVGQWNKFR DEVEDDYFRT WNPGKPFDQA LDPAKDPCLK TKCSRHKVCI TQDAQTALCI SHRRLTHSMK EVGGSHKQWR GLPSSTCKPC PIAYASPVCG SDGHSYSSQC KLEYQACVLG KQISIKCEGR CPCPSDKSMN IGRNVKRACS DLEFREVANR LRDWFKALHE SGSQNKKTKA LLRPERSRFD TSILPICKDS LGWMFNRLDT NYDLLLDQSE LGSIYLDKNE QCTKAFFNSC DTYKDSLISN NEWCYCFQRQ QDPPCHTELS NIQKRQGIKK LLGQYIPLCD EDGYYKPTQC HGSVGQCWCV DRYGNEVVGS RINGVADCAI DFEISGDFAS GDFREWTDDE GEEDDIMNDK DDIEDDDEDE GDDDDDGDVH DGYIVDHHHH HH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
DisclaimerFor research use only

Alternative Names

Testican-3; SPARC/osteonectin CWCV and Kazal-like domains proteoglycan 3

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse SPOCK3 protein (orb392515)

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Available Sizes

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10 μg
$ 250.00
50 μg
$ 600.00
500 μg
$ 3,860.00
DispatchUsually dispatched within 5-10 working days
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