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| Catalog Number | orb428438 |
|---|---|
| Category | Proteins |
| Description | Recombinant of mouse SHH protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM Na2PO4 pH-7.5. |
| Purity | Greater than 97.0% as determined by SDS-PAGE. |
| Protein Sequence | MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
| Application notes | Cytokines And Growth Factors |
| Source | Escherichia Coli |
| Biological Activity | Determined by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.48-0.72 µg/ml. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Sonic Hedgehog in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
| Alternative names | SHH, HHG-1, HHG1, Sonic hedgehog protein. |
| Note | For research use only |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 20.4 KDa. Observed: 22 KDa, reducing conditions |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 19.8 KDa. Observed: 18-22 KDa, reducing conditions |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 19.8 KDa. Observed: 20 KDa, reducing conditions |
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