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Catalog Number | orb392441 |
---|---|
Category | Proteins |
Description | Recombinant mouse SEMA4D protein is produced by our mammalian expression system and the target gene encoding Phe24-Met711 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SEMA4D |
Entrez | 20354 |
UniProt ID | O09126 |
Protein Sequence | FAPVPRLTWE HGEVGLVQFH KPGIFNYSAL LMSEDKDTLY VGAREAVFAV NALNISEKQH EVYWKVSEDK KSKCAEKGKS KQTECLNYIR VLQPLSSTSL YVCGTNAFQP TCDHLNLTSF KFLGKSEDGK GRCPFDPAHS YTSVMVGGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIQKSPDG PEGEDDKVYF FFTEVSVEYE FVFKLMIPRV ARVCKGDQGG LRTLQKKWTS FLKARLICSK PDSGLVFNIL QDVFVLRAPG LKEPVFYAVF TPQLNNVGLS AVCAYTLATV EAVFSRGKYM QSATVEQSHT KWVRYNGPVP TPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPKL IKKDVNYTQI VVDRTQALDG TFYDVMFIST DRGALHKAVI LTKEVHVIEE TQLFRDSEPV LTLLLSSKKG RKFVYAGSNS GVVQAPLAFC EKHGSCEDCV LARDPYCAWS PAIKACVTLH QEEASSRGWI QDMSGDTSSC LDKSKESFNQ HFFKHGGTAE LKCFQKSNLA RVVWKFQNGE LKAASPKYGF VGRKHLLIFN LSDGDSGVYQ CLSEERVRNK TVSQLLAKHV LEVKMVPRTP PSPTSEDAQT EGSKITSKMP VASTQGSSPP TPALWATSPR AATLPPKSSS GTSCEPKMVD DIEGRMDEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; G Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Sema4d(Phe24-Met711) was fused with the C-terminal Fc Tag and expressed in Mammalian cells. |
98.00% | |
110-130 KDa, reducing conditions |
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