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Catalog Number | orb427713 |
---|---|
Category | Tools |
Description | Recombinant of mouse PKM2 protein |
Form/Appearance | Sterile Filtered clear solution. |
Purity | Greater than 85% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP |
Source | Escherichia Coli |
Biological Activity | Specific activity: > 500 units/mg. One unit will convert 1 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles |
Buffer/Preservatives | PKM2 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH 8.5), 0.2M NaCl, 1mM DTT and 30% glycerol. |
Alternative names | Pyruvate kinase isozymes M1/M2, EC 2.7.1.40, Pyruv Read more... |
Note | For research use only |
Application notes | Protein Kinases |
Expiration Date | 12 months from date of receipt. |
IF, WB | |
Human, Mouse, Primate, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IP, WB | |
Human, Mouse, Primate | |
Mouse | |
Monoclonal | |
Unconjugated |
Greater than 85% as determined by SDS-PAGE. | |
62.8 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
61.8 kDa | |
Baculovirus |
Greater than 90% as determined by SDS-PAGE. | |
61.8 kDa | |
Mammalian cell |
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