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Mouse PEDF protein

SKU: orb392467

Description

Recombinant mouse PEDF protein is produced by our E. coli expression system and the target gene encoding Asp43-Thr417 is expressed.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityMouse
Protein SequenceMDPFFKVPVN KLAAAVSNFG YDLYRLRSSA SPTGNVLLSP LSVATALSAL SLGAEHRTES VIHRALYYDL ITNPDIHSTY KELLASVTAP EKNLKSASRI VFERKLRVKS SFVAPLEKSY GTRPRILTGN PRVDLQEINN WVQAQMKGKI ARSTREMPSA LSILLLGVAY FKGQWVTKFD SRKTTLQDFH LDEDRTVRVP MMSDPKAILR YGLDSDLNCK IAQLPLTGSM SIIFFLPLTV TQNLTMIEES LTSEFIHDID RELKTIQAVL TVPKLKLSFE GELTKSLQDM KLQSLFESPD FSKITGKPVK LTQVEHRAAF EWNEEGAGSS PSPGLQPVRL TFPLDYHLNQ PFLFVLRDTD TGALLFIGRI LDPSST
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
DisclaimerFor research use only

Alternative Names

PEDF; Pigment epithelium-derived factor; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse PEDF protein (orb392467)

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Available Sizes

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10 μg
$ 260.00
50 μg
$ 600.00
500 μg
$ 3,450.00
DispatchUsually dispatched within 5-10 working days
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