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Mouse PEDF protein

SKU: orb392467

Description

Recombinant mouse PEDF protein is produced by our E. coli expression system and the target gene encoding Asp43-Thr417 is expressed.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetSERPINF1
ReactivityMouse
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMDPFFKVPVN KLAAAVSNFG YDLYRLRSSA SPTGNVLLSP LSVATALSAL SLGAEHRTES VIHRALYYDL ITNPDIHSTY KELLASVTAP EKNLKSASRI VFERKLRVKS SFVAPLEKSY GTRPRILTGN PRVDLQEINN WVQAQMKGKI ARSTREMPSA LSILLLGVAY FKGQWVTKFD SRKTTLQDFH LDEDRTVRVP MMSDPKAILR YGLDSDLNCK IAQLPLTGSM SIIFFLPLTV TQNLTMIEES LTSEFIHDID RELKTIQAVL TVPKLKLSFE GELTKSLQDM KLQSLFESPD FSKITGKPVK LTQVEHRAAF EWNEEGAGSS PSPGLQPVRL TFPLDYHLNQ PFLFVLRDTD TGALLFIGRI LDPSST

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PEDF; Pigment epithelium-derived factor; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse PEDF protein (orb392467)

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Available Sizes

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10 μg
$ 260.00
50 μg
$ 600.00
500 μg
$ 3,450.00
DispatchUsually dispatched within 5-10 working days
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