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Description
Research Area
Metabolism Research
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | PCSK9 |
| Reactivity | Mouse |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | QDEDGDYEEL MLALPSQEDG LADEAAHVAT ATFRRCSKEA WRLPGTYIVV LMEETQRLQI EQTAHRLQTR AARRGYVIKV LHIFYDLFPG FLVKMSSDLL GLALKLPHVE YIEEDSFVFA QSIPWNLERI IPAWHQTEED RSPDGSSQVE VYLLDTSIQG AHREIEGRVT ITDFNSVPEE DGTRFHRQAS KCDSHGTHLA GVVSGRDAGV AKGTSLHSLR VLNCQGKGTV SGTLIGLEFI RKSQLIQPSG PLVVLLPLAG GYSRILNAAC RHLARTGVVL VAAAGNFRDD ACLYSPASAP EVITVGATNA QDQPVTLGTL GTNFGRCVDL FAPGKDIIGA SSDCSTCFMS QSGTSQAAAH VAGIVARMLS REPTLTLAEL RQRLIHFSTK DVINMAWFPE DQQVLTPNLV ATLPPSTHET GGQLLCRTVW SAHSGPTRTA TATARCAPEE ELLSCSSFSR SGRRRGDWIE AIGGQQVCKA LNAFGGEGVY AVARCCLVPR ANCSIHNTPA ARAGLETHVH CHQKDHVLGC SFHWEVEDLS VRRQPALRSR RQPGQCVGHQ AASVYASCCH APGLECKIKE HGISGPSEQV TVACEAGWTL TGCNVLPGAS LTLGAYSVDN LCVARVHDTA RADRTSGEAT VAAAICCRSR PSAKASWVQH HHHHH |
Storage & Handling
−| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NARC1; Proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Mouse PCSK9 protein (orb392244)
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