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Catalog Number | orb392210 |
---|---|
Category | Proteins |
Description | Recombinant mouse NT5E protein is produced by our mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus. |
Target | NT5E |
Reactivity | Mouse |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVAYAFGKYL GYLKVEFDDK GNVITSYGNP ILLNSSIPED ATIKADINQW RIKLDNYSTQ ELGRTIVYLD GSTQTCRFRE CNMGNLICDA MINNNLRHPD EMFWNHVSMC IVNGGGIRSP IDEKNNGTIT WENLAAVLPF GGTFDLVQLK GSTLKKAFEH SVHRYGQSTG EFLQVGGIHV VYDINRKPWN RVVQLEVLCT KCRVPIYEPL EMDKVYKVTL PSYLANGGDG FQMIKDELLK HDSGDQDISV VSEYISKMKV VYPAVEGRI KFHHHHHH |
UniProt ID | Q61503 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Alternative names | NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleoti Read more... |
Note | For research use only |
Entrez | 23959 |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Greater than 95% as determined by SDS-PAGE. | |
58.8 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse NT5E (NP_0359811) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |