Mouse NT5E protein

SKU: orb392210

Description

Recombinant mouse NT5E protein is produced by our mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: NT5E
• Reactivity: Mouse
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVAYAFGKYL GYLKVEFDDK GNVITSYGNP ILLNSSIPED ATIKADINQW RIKLDNYSTQ ELGRTIVYLD GSTQTCRFRE CNMGNLICDA MINNNLRHPD EMFWNHVSMC IVNGGGIRSP IDEKNNGTIT WENLAAVLPF GGTFDLVQLK GSTLKKAFEH SVHRYGQSTG EFLQVGGIHV VYDINRKPWN RVVQLEVLCT KCRVPIYEPL EMDKVYKVTL PSYLANGGDG FQMIKDELLK HDSGDQDISV VSEYISKMKV VYPAVEGRI KFHHHHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73

Quick Database Links

Gene Symbol

NT5E

UniProt

Q61503

NCBI Gene ID

23959