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Catalog Number | orb392210 |
---|---|
Category | Proteins |
Description | Recombinant mouse NT5E protein is produced by our mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | NT5E |
Entrez | 23959 |
UniProt ID | Q61503 |
Protein Sequence | WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVAYAFGKYL GYLKVEFDDK GNVITSYGNP ILLNSSIPED ATIKADINQW RIKLDNYSTQ ELGRTIVYLD GSTQTCRFRE CNMGNLICDA MINNNLRHPD EMFWNHVSMC IVNGGGIRSP IDEKNNGTIT WENLAAVLPF GGTFDLVQLK GSTLKKAFEH SVHRYGQSTG EFLQVGGIHV VYDINRKPWN RVVQLEVLCT KCRVPIYEPL EMDKVYKVTL PSYLANGGDG FQMIKDELLK HDSGDQDISV VSEYISKMKV VYPAVEGRI KFHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
Alternative names | NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleoti Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
58.8 kDa | |
Mammalian cell |
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