Mouse NOTCH1 Protein

• Catalog Number: orb653659
• Category: Proteins
• Description: NOTCH1 Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.
• Reactivity: Mouse
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 55.4 kDa
• Target: NOTCH1
• Protein Sequence: NP_032740.3
• Source: Mouse NOTCH1, His Tag (orb653659) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Gln 526 (Accession # Q01705-1).
• Expression System: HEK293
• Expression Region: Ala 19 - Gln 526
• Endotoxins: 1.0 EU per μg
• NCBI: NP_032740.3
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: NOTCH1,Notch 1,hN1,TAN1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.4 kDa. The protein migrates as 58-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.