Notch1 Antibody

SKU: orb99099

Description

Mouse Anti-Mouse Notch1 Monoclonal IgG1

Research Area

Cardiovascular Research, Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N253/32 (Formerly sold as S253-32)
• Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
• Target: Notch1
• Molecular Weight: 270kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurogenic locus notch homolog protein 1, hN1, Translocation associated notch protein, TAN1, TAN-1, Motch A, mT14, p300, Motch, Mis6, Neurogenic locus notch protein homolog, NICD, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH Drosophila, homolog of 1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, xotch

Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone N253/32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.

Quick Database Links

Gene Symbol

Notch1

UniProt

Q01705

NCBI Gene ID

18128

RefSeq (Protein):NP_032740.3