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Catalog Number | orb80093 |
---|---|
Category | Proteins |
Description | Mouse Noggin protein |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by SDS-PAGE. |
Conjugation | Unconjugated |
Target | Noggin |
Solubility (25°C) | It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. |
Protein Sequence | MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGQRCGW IPIQYPIISE CKCSC. |
Biological Activity | The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of NOGGIN. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.2 um filtered solution in 30% CH3CN, 0.1% TFA. |
Alternative names | Nog Protein, NOGG_HUMAN Protein, Noggin Protein Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
49.2 kDa | |
Human Noggin Protein, Fc Tag, premium grade (orb257715) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Cys 232 (Accession # Q13253-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 95% by SDS-PAGE. | |
KMP1876, Recombinant Mouse Noggin Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of mouse Noggin (Accession #NP_032737.1) fused with an 6×His Tag at the C-terminus. |
29-31 kDa, observed by reducing SDS-PAGE. | |
CHO |
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