(Mouse) Med15 Antibody (C-term)

• Catalog Number: orb1788004
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Immunogen: 712-746 aa
• UniProt ID: Q924H2
• MW: 86607 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:25
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Med15 Antibody (C-term) at 1:1000 dilution. Lane 1: mouse brain lysates. Lane 2: mouse spleen lysates. Lane 3: mouse testis lysates. Lane 4: mouse thymus lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.