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Catalog Number | orb426986 |
---|---|
Category | Proteins |
Description | Recombinant of mouse MANF protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10µg/ml, corresponding to a specific activity of > 100 IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MANF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Mesencephalic astrocyte-derived neurotrophic facto Read more... |
Note | For research use only |
Mouse | |
31.25-2000pg/ml | |
18.75pg/ml |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses. | |
18.2 kDa |
Mouse | |
15.6-1000pg/mL | |
7.7pg/mL |
> 90%, determined by SDS-PAGE | |
This protein contains the mouse MANF (NP_0833792) (Met 1-Leu 179) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |
0.16-10 ng/mL | |
0.047 ng/mL |