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Catalog Number | orb168697 |
---|---|
Category | Proteins |
Description | Recombinant of Mouse KGF protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-8 and 0.1M NaCl. |
Purity | Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
Protein Sequence | MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 100,000 Units/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized KGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Alternative names | HBGF-7, FGF7, FGF-7, KGF, Keratinocyte growth fact Read more... |
Note | For research use only |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% pure by SDS-PAGE and HPLC analyses. | |
19.3 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.8 kDa | |
E.Coli |
> 90%, determined by SDS-PAGE | |
This protein contains the mouse FGF7 (NP_0320341) (Cys32-Thr194) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli. |
> 96% by SDS-PAGE and HPLC analyses. | |
Approximately 19.3 kDa, 170 amino acid residues consisting of Methionine and the mature human KGF-2 (amino acid residues 40 – 208). | |
Escherichia coli. |