| Category | Proteins |
|---|---|
| Catalog Number | orb391909 |
| Reactivity | Mouse |
| Tested applications | ELISA, SDS-PAGE, WB, MS |
| Target | IL10 |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Protein Sequence | MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S |
| Source | E. coli |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
| Endotoxin | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Note | For research use only. |
| Expiration Date | 6 months from date of receipt. |
| Description | Recombinant mouse IL10 protein is produced by our E. coli expression system and the target gene encoding Ser19-Ser178 is expressed. |
- 1 imageMouse IL10 protein [orb80068]
HPLC, FA, SDS-PAGE
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Unconjugated
2 μg, 10 μg, 1 mg, 100 μg, 250 μg, 500 μg -
- 1 imageMouse IL10 protein (Active) [orb359015]
E.Coli
18.7 kDa
> 97% as determined by SDS-PAGE and HPLC.
10 μg, 100 μg, 500 μg -
- Mouse IL-10 protein [orb1291972]
ELISA, WB
E.Coli
39.4 kDa
Greater than 95% by SDS-PAGE gel analyses
50 μg, 100 μg, 200 μg, 1 mg - Mouse IL-10 protein [orb754238]
ELISA, WB
E.Coli
17.5 kDa
Greater than 95% as determined by SDS-PAGE
50 μg, 200 μg, 1 mg, 100 μg
