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Mouse IL1 alpha protein
Catalog Number: orb80049
| Catalog Number | orb80049 |
|---|---|
| Category | Proteins |
| Description | Mouse IL1 alpha protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | Mouse IL1 alpha |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 1a in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Tyr-Thr. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine D10S cells is 0.01 ng/ml, corresponding to Specific Activity of 100,000,000 IU/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) sterile solution containing 1X PBS, pH=7.4. |
| Alternative names | Hematopoietin 1 protein, Hematopoietin-1 protein, Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.44 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL1 alpha protein
- Item 1 of 1
Mouse IL1 alpha protein (Active) [orb359005]
> 97% as determined by SDS-PAGE and HPLC.
17.9 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human IL1A Protein, mFc Tag [orb2321373]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of IL1A-mFc is approximately 35-70 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg 
Mouse IL1 alpha protein [orb391944]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
E. coli
500 μg, 50 μg, 10 μg- Item 1 of 1
Recombinant mouse IL-1 Alpha protein, His (HEK293) [orb1516667]
> 95% as determined by SDS-PAGE
24 kDa
500 μg, 100 μg Mouse IL1 Receptor protein [orb707227]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μg
