Mouse IL1 alpha protein

SKU: orb391944

Description

Recombinant mouse IL1 alpha protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 156 amino acids with a molecular mass of 18000 Daltons.

Research Area

Epigenetics, Immunology, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Mouse
• Protein Sequence: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
• Disclaimer: For research use only

Alternative Names

IL1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1

Quick Database Links

UniProt

P01582

NCBI Gene ID

16175