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Catalog Number | orb750344 |
---|---|
Category | Proteins |
Description | Mouse IL-6, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1). |
Target | IL-6 |
Conjugation | Unconjugated |
Tag | N-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_112445.1 |
UniProt ID | P08505 |
MW | 23.6 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-6, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha, His, (Cat. No. ILR-M82E9) with a linear range of 20-156 ng/mL (QC tested). |
Expression Region | Phe 25 - Thr 211 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_112445.1 |
Immobilized Mouse IL-6, His Tag (Cat. No. orb750344) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha, His, Avitag with a linear range of 20-156 ng/mL (QC tested).
98.00% | |
56.3 kDa (predicted). Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. |