Mouse IL-5 Protein

• Catalog Number: orb762422
• Category: Proteins
• Description: Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease. Drugs that target IL-5 are mepolizumab and reslizumab.
• Reactivity: Mouse
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 15.0 KDa
• Target: IL-5
• Source: Mouse IL-5, His Tag (orb762422) is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1).
• Expression System: HEK293
• Biological Activity: Immobilized Mouse IL-5, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).
• Expression Region: Met 21 - Gly 133
• Endotoxins: 1.0 EU per μg
• NCBI: NP_034688.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL-5,TRF,IL5,Interleukin-5
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 KDa. The protein migrates as 15 kDa,19 kDa and 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Mouse IL-5, His Tag (Cat. No. orb762422) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).